01
|
Write
a description of the principles of Gas Chromatography (GC) as a separation
tool (see L6.01). |
02
|
Identify
uses of GC as an analytical tool. |
03
|
Identify
the components of a gas chromatograph. |
04
|
Relate
retention time and resolution to the characterization of mixtures. |
05
|
Identify
detectors, including thermal conductivity, flame ionization, and electron
capture in terms of their use, detection limits, and special characteristics;
list advantages and disadvantages of each. |
06
|
Relate
the effects of column length to separation. |
07
|
Install
and maintain a variety of chromatographic columns, including capillary,
glass, etc. |
08
|
Identify
and describe characteristics of a variety of column types in terms of packing
material and construction material, with emphasis on packed vs. capillary
columns. |
09
|
Use
a variety of gas chromatographs to analyze known and unknown mixtures
by applying the following procedures:
- prepare samples
- dilute samples
as appropriate
- select column
packing
- choose injection
temperature
- choose column
length
- adjust flow
- condition columns
- calibrate instrument
- optimize conditions
- identify the
components in a mixture
- calculate the
percentage of the components in the mixture
- repeat the analysis
- calculate the
precision of the measurement
|
10
|
Identify
and describe a variety of column packings in terms of their polarity on
phases and describe when to use each. |
11
|
Characterize
a variety of injection ports and the usefulness of each for gases and liquids. |
12
|
Operate
a computer-controlled gas chromatograph. |
13
|
Identify
major vendors of gas chromatographic instruments. |
14
|
Provide
examples of at least six applications of GC in local industries. |